Melamine ELISA Test Kit (China)

Principle



The MelamineELISA Test Kit is a competitive enzyme-labeled

immunoassay. Melamine is extracted from a sample by blending or

shaking with extraction solution. The Melamine HRP conjugate, sample

extract and calibrators are pipetted into the test wells coated with

Melamine antibody to initiate the reaction. During the 30 minute

incubation period, Melamine from the sample and Melamine HRP

conjugate compete for binding to Melamine antibody. Following this

30 minute incubation, the contents of the well are removed and the

wells are washed to remove any unbound Melamine, and Melamine HRP

conjugate. After wash with laboratory grade water, a clear

substrate is then added to the wells and any bound enzyme conjugate

causes the conversion to a blue color. Following a 30 minute

incubation, the reaction is stopped and the amount of color in each

well is read. The color of the unknown samples is compared to the

color of the calibrators and the Melamine concentration of the

samples is derived.

Specifications



Plate containing 12 test strips of 8 wells each vacuum-packed in

aluminized pouch with indicating dessicant.

4 vials each containing 2 mL of Melamine calibrators corresponding

to 0, 20, 100 and 500 µg/L (ppb) of Melamine.

1 vial containing 7 mL Melamine HRP Enzyme Conjugate.

1 vial containing 14 mL of Substrate.

1 vial containing 14 mL of Stop Solution. (Caution! 1N HCl. Handle

with care.)