Benzyl penicillin test Kit (China)

Brief



Benzyl penicillin is widely applicable antibiotic, which is commonly

employed in prevention, therapy, diagnosis of animal disease, or

adjustment of physiological functions. Benzyl penicillin is

synthesized to control animal disease effectively and promote the

healthy development of farming industry. If animals absorb it too

much, will cause Bp-residues in animal food. Mankind produces

allergic reaction and tolerance to a drug resulting from taking high

residues food. Use of Benzyl penicillin was resitricted in the EU

and America. The EU regulated that maximum residue in milk is 4 ppb,

Chinese Agriculture Ministry made corresponding national policies

restrict its application.

Principle



The GreenSpring™ Benzyl penicillin (Bp) ELISA Test Kit is based on

the competitive enzyme immunoassay for the detection of Benzyl

penicillin in the tissue(chicken, pork, duck), honey, milk, fish,

shrimp and egg. The coupling antigen is pre-coated on the micro-well

stripes. The Benzyl penicillin in the testing sample competes with

the coupling antigens pre-coated on the micro-well stripes for the

antibodies against Benzyl penicillin. After the addition of the

enzyme conjugate, the TMB substrate is added for coloration. The

optical density (OD) value of the testing sample has a negative

correlation with the content of Benzyl penicillin in it. This value

is compared to the standard curve and the content of the

corresponding Benzyl penicillin is subsequently obtained.

Specifications



Sensitivity : 0.1ppb

Detection limit:

Chicken, duck, pork, fish, shrimp 2ppb

Honey, milk 2ppb

Recovery rate

Chicken, duck, pork, fish, shrimp 85%±10%

Honey , milk 70%±10%

Cross-reaction rate:

Benzyl penicillin 100%

Ampicillin 0.8%

Cloxacillin 0.2%

Dicloxacillin 0.1%

Amoxicillin 0.1%

Ceftiofur 0.1%