Neomycin ELISA test Kit (China)

Brief



Neomycin, an antibiotic, is used to prevent or treat skin infections

caused by bacteria. It is not effective against fungal or viral

infections. Neomycin may cause damage to the kidneys and/or nerves.

It’s potentially toxic compounds causing significant damage in

vestibular andauditory functions in humans as well as in animals.

The GreenSpring™ Neomycin ELISA test Kit is very simple in sample

pre-treatment, which only need 10 minutes.

Principle



This test kit is based on the competitive enzyme immunoassay. The

coupling antigen is pre-coated on the micro-well stripes. The

Neomycin residues in the sample and the coupling antigen pre-coated

on the micro-well stripes compete for the anti-Neomycin antibody.

After the addition of the enzyme conjugate, the TMB substrate is

added for coloration. The optical density (OD) value of the sample

has a negative correlation with the Neomycin in it. This value is

compared to the standard curve and the Neomycin concentration is

subsequently obtained.

Specifications



Sensitivity: 0.5 ppb

Detection limit

Honey.......10 ppb

Chicken/liver, pork/liver, fish,

shrimp... 10 ppb

Recovery rate

Chicken, pork, honey, fish,

shrimp...... 85±15%

Liver(chicken or

porcine)..... 70±10%

Cross-reaction rate

Neomycin......100%

Streptomycin.......< 0.1%

Gentamicin.......< 0.1%

Dihydrostreptomycin....< 0.1%

Kanamycin......< 0.1%

Tobramycin.....< 0.1%

Sisomicin......< 0.1%