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Principle
This test kit is based on the competitive enzyme immunoassay for the
detection of Tylosin in the meat and liver(chicken, pork), honey. The
enzyme conjugate is pre-coated on the micro-well stripes. The
Chloramphenicol in the testing sample competes with the coupling
antigen pre-coated on the micro-well stripes for the antibody
against Tylosin. After the addition of the enzyme conjugate, the TMB
substrate is added for coloration. The optical density (OD) value of
the testing sample has a negative correlation with the content of
Tylosin in it. This value is compared to the standard curve and the
content of the corresponding Tylosin is subsequently obtained.
Specifications
Sensitivity : 1.5 ppb
Detection limit:
Meat 3 ppb
Liver 3 ppb
Honey 1.5 ppb
Recovery rate
Meat 80%±15%
Liver 75%±10%
Honey 80%±15%