Diazepam ELISA Test Kit (China)

Principle

This test kit is based on the competitive enzyme immunoassay for the

detection of Diazepam in the feed,urine, liver, meat. The coupling

antigen is pre-coated on the micro-well stripes. The Diazepam in the

testing sample competes with the coupling antigen pre-coated on the

micro-well stripes, to interact with the antibodies against

Diazepam. After the addition of the enzyme conjugate, the TMB

substrate is added for coloration. The optical density (OD) value of

the testing sample has a negative correlation with the content of

Diazepam in the sample. This value is compared to the standard curve

and the content of the corresponding Diazepam is subsequently

obtained.

Specifications

Sensitivity : 0.1ppb

Detection limit

Animal tissue 1ppb

Urine 1ppb

Compound feed 10ppb

Concentrated feed 20ppb

Premixed feed 20ppb

Recovery rate

Urine 80 ±10%

Feed 75 ±10%

Meat,liver(pork) 75 ±10%

Cross-reactions rate

DiaZepatn 100%

Nitrazepam 7.6%

Oxazepam 8.8%